Editorial 163
Spotlight on Three Rs Progress 165
Resources Round-up 168
Obituary
Dr Robert Combes (1946–2021) 171
Comments
Zebrafish Beyond the Bench: The ‘Plataforma Zebrafish Open Doors’ Programme 175
Geonildo Rodrigo Disner, Maria Alice Pimentel Falcão, Carla Lima and Monica Lopes-Ferreira
It’s Time to Include Harm to Humans in Harm–Benefit Analysis — But How to Do It, That is the Question 182
Michael Balls
Review Article
A Systematic Review of Published Physiologically-based Kinetic Models and an Assessment of their Chemical Space Coverage 197
Courtney V. Thompson, James W. Firman, Michael R. Goldsmith, Christopher M. Grulke, Yu-Mei Tan, Alicia Paini, Peter E. Penson, Risa R. Sayre, Steven Webb and Judith C. Madden
Research Article
Comparison of Long-term Human Precision-cut Lung Slice Culture Methodology and Response to Challenge:An Argument for Standardisation 209
Vivek Patel, Khalid Amin, David Allen, Lindsey Ukishima, Adam Wahab, Chad Grodi and Holger Behrsing
Conference Diary 223